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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant position in marketing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually described. Two extra splice variants were being reported but in depth nucleotide sequence data is not yet out there.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.
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This information outlines even more feasible optimization of mobile and immunoassay parameters to make certain the absolute best success are...
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This guidebook outlines even more attainable optimization of cellular and immunoassay parameters to be sure the absolute best results are...
Antibodies certain to the blocking peptide no more bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody get more info by yourself, one can see which staining is particular.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which lead to the temporal coordination of each mitotic party. Cyclin E types a posh with and features for a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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